Description: Rabbit polyclonal antibody to PRPF19 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRPF19. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PRPF19 Alternative Names: NMP200; PRP19; SNEV; Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19/PSO4 homolog; hPso4; Senescence evasion factorEntrez Gene (Human): 27339Entrez Gene (Mouse) : 28000Entrez Gene (Rat) : 246216SwissProt (Human): Q9UMS4SwissProt (Mouse) : Q99KP6SwissProt (Rat) : Q9JMJ4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PRPF19 expression in HEK293T (A), Hela (B), MGC (C), mouse brain (D), mouse kidney (E), rat kidney (F) whole cell lysates.
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Immunohistochemical analysis of PRPF19 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.