Description: Rabbit polyclonal antibody to HBP1 (Phospho-S402) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S402 of human HBP1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HBP1 Alternative Names: HMG box-containing protein 1; HMG box transcription factor 1; High mobility group box transcription factor 1Entrez Gene (Human): 26959Entrez Gene (Mouse) : 73389Entrez Gene (Rat) : 27080SwissProt (Human): O60381SwissProt (Mouse) : Q8R316SwissProt (Rat) : Q62661Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HBP1 (Phospho-S402) expression in HEK293T PMA-treated (A), mouse spleen (B), rat spleen (C) whole cell lysates.
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Immunohistochemical analysis of HBP1 (Phospho-S402) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.