Description: Rabbit polyclonal antibody to GRASP55 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GRASP55. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GORASP2 Alternative Names: GOLPH6; Golgi reassembly-stacking protein 2; GRS2; Golgi phosphoprotein 6; GOLPH6; Golgi reassembly-stacking protein of 55 kDa; GRASP55; p59Entrez Gene (Human): 26003Entrez Gene (Mouse) : 70231SwissProt (Human): Q9H8Y8SwissProt (Mouse) : Q99JX3SwissProt (Rat) : Q9R064Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GRASP55 expression in Hela (A), HGC27 (B), mouse testis (C), rat liver (D), rat testis (E) whole cell lysates.
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Immunohistochemical analysis of GRASP55 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.