Description: Rabbit polyclonal antibody to BRMS1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRMS1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: BRMS1 Alternative Names: Breast cancer metastasis-suppressor 1Entrez Gene (Human): 25855Entrez Gene (Mouse) : 107392Entrez Gene (Rat) : 100911841; 293668SwissProt (Human): Q9HCU9SwissProt (Mouse) : Q99N20SwissProt (Rat) : Q5M7T3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BRMS1 expression in HT29 (A), HeLa (B), MCF7 (C) whole cell lysates.
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Immunohistochemical analysis of BRMS1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules