Description: Rabbit polyclonal antibody to SHCB Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SHCB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SHC2 Alternative Names: SCK; SHCB; SHC-transforming protein 2; Protein Sck; SHC-transforming protein B; Src homology 2 domain-containing-transforming protein C2; SH2 domain protein C2Entrez Gene (Human): 25759Entrez Gene (Mouse) : 216148Entrez Gene (Rat) : 314612SwissProt (Human): P98077SwissProt (Mouse) : Q8BMC3SwissProt (Rat) : O70142Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SHCB expression in mouse testis (A), rat testis (B) whole cell lysates.
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Immunohistochemical analysis of SHCB staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.