Description: Rabbit polyclonal antibody to EDG7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EDG7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: LPAR3 Alternative Names: EDG7; LPA3; Lysophosphatidic acid receptor 3; LPA receptor 3; LPA-3; Lysophosphatidic acid receptor Edg-7Entrez Gene (Human): 23566SwissProt (Human): Q9UBY5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EDG7 expression in HepG2 (A), NT2D1 (B) whole cell lysates.
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Immunohistochemical analysis of EDG7 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.