Description: Rabbit polyclonal antibody to ANP32D Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ANP32D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ANP32D Alternative Names: PP32R2; Acidic leucine-rich nuclear phosphoprotein 32 family member D; Phosphoprotein 32-related protein 2; Tumorigenic protein pp32r2Entrez Gene (Human): 23519SwissProt (Human): O95626Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ANP32D expression in HeLa (A), mouse liver (B), rat liver (C) whole cell lysates.
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Immunohistochemical analysis of ANP32D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.