Description: Rabbit polyclonal antibody to ABCA5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ABCA5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ABCA5 Alternative Names: KIAA1888; ATP-binding cassette sub-family A member 5Entrez Gene (Human): 23461Entrez Gene (Mouse) : 217265Entrez Gene (Rat) : 286970SwissProt (Human): Q8WWZ7SwissProt (Mouse) : Q8K448SwissProt (Rat) : Q8CF82Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ABCA5 expression in human testis (A) whole cell lysates.
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Immunohistochemical analysis of ABCA5 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.