Description: Rabbit polyclonal antibody to RGL1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RGL1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RGL1 Alternative Names: KIAA0959; RGL; Ral guanine nucleotide dissociation stimulator-like 1; RalGDS-like 1Entrez Gene (Human): 23179Entrez Gene (Mouse) : 19731SwissProt (Human): Q9NZL6SwissProt (Mouse) : Q60695Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of RGL1 expression in 3T3L1 (A), H9C2 (B), PC3 (C), LOVO (D), HCT116 (E) whole cell lysates.
-
Immunohistochemical analysis of RGL1 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.