Description: Rabbit polyclonal antibody to RASA3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RASA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RASA3 Alternative Names: Ras GTPase-activating protein 3; GAP1(IP4BP); Ins P4-binding proteinEntrez Gene (Human): 22821Entrez Gene (Mouse) : 19414Entrez Gene (Rat) : 29372SwissProt (Human): Q14644SwissProt (Mouse) : Q60790SwissProt (Rat) : Q9QYJ2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RASA3 expression in HEK293T (A), A549 (B), H1792 (C), mouse liver (D), mouse heart (E), rat liver (F), rat heart (G) whole cell lysates.
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Immunohistochemical analysis of RASA3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.