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Anti-DJ-1 Antibody CPA2547
  • Western blot analysis of DJ-1 expression in HepG2 (A), mouse kidney (B), mouse liver (C), rat kidney (D), rat liver (E) whole cell lysates.
  • Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-DJ-1 Antibody
Cat No: CPA2547
Source: Rabbit
Reactivity: H, M, R, Mk
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to DJ-1
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DJ-1. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol: PARK7
Alternative Names: Protein DJ-1; Oncogene DJ1; Parkinson disease protein 7
Entrez Gene (Human): 11315
Entrez Gene (Rat) : 117287
SwissProt (Human): Q99497
SwissProt (Rat) : O88767
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of DJ-1 expression in HepG2 (A), mouse kidney (B), mouse liver (C), rat kidney (D), rat liver (E) whole cell lysates.
  • Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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