Description: Rabbit polyclonal antibody to RPL35 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL35. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RPL35 Alternative Names: 60S ribosomal protein L35Entrez Gene (Human): 11224Entrez Gene (Mouse) : 66489Entrez Gene (Rat) : 296709SwissProt (Human): P42766SwissProt (Mouse) : Q6ZWV7SwissProt (Rat) : P17078Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RPL35 expression in U2OS (A), HeLa (B), NIH3T3 (C) whole cell lysates.
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  Immunohistochemical analysis of RPL35 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.