Description: Rabbit polyclonal antibody to KAT7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human KAT7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/100) Gene Symbol: KAT7 Alternative Names: HBO1; HBOa; MYST2; Histone acetyltransferase KAT7; Histone acetyltransferase binding to ORC1; Lysine acetyltransferase 7; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST-2Entrez Gene (Human): 11143Entrez Gene (Mouse) : 217127Entrez Gene (Rat) : 303470SwissProt (Human): O95251SwissProt (Mouse) : Q5SVQ0SwissProt (Rat) : Q810T5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of KAT7 expression in HepG2 (A), MCF7 (B) whole cell lysates.
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Immunofluorescent analysis of KAT7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Exchange of associated factors directs a switch in HBO1 acetyltransferase histone tail specificity
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