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Anti-KAT7 Antibody CPA2534
  • Western blot analysis of KAT7 expression in HepG2 (A), MCF7 (B) whole cell lysates.
  • Immunofluorescent analysis of KAT7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Product NameAnti-KAT7 Antibody
Cat No: CPA2534
Source: Rabbit
Reactivity: H, M, R, B, C, D, P, Z
Applications: WB, IF/IC, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to KAT7
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human KAT7. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/100)
Gene Symbol: KAT7
Alternative Names: HBO1; HBOa; MYST2; Histone acetyltransferase KAT7; Histone acetyltransferase binding to ORC1; Lysine acetyltransferase 7; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST-2
Entrez Gene (Human): 11143
Entrez Gene (Mouse) : 217127
Entrez Gene (Rat) : 303470
SwissProt (Human): O95251
SwissProt (Mouse) : Q5SVQ0
SwissProt (Rat) : Q810T5
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of KAT7 expression in HepG2 (A), MCF7 (B) whole cell lysates.
  • Immunofluorescent analysis of KAT7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Exchange of associated factors directs a switch in HBO1 acetyltransferase histone tail specificity
Journal Genes Dev
IF 10.5
Application
Reactivity
PMID 24065767
SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition complex (HBO1) ubiquitin-proteasomal degradation to regulate cell proliferation
Journal J Biol Chem
IF 4.8
Application WB
Reactivity Mouse
PMID 23319590
An expanded Oct4 interaction network: implications for stem cell biology, development, and disease
Journal Cell Stem Cell
IF 23.9
Application WB
Reactivity Mouse
PMID 20362542
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