Description: Rabbit polyclonal antibody to FRS3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FRS3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FRS3 Alternative Names: Fibroblast growth factor receptor substrate 3; FGFR substrate 3; FGFR-signaling adaptor SNT2; Suc1-associated neurotrophic factor target 2; SNT-2Entrez Gene (Human): 10817Entrez Gene (Mouse) : 107971Entrez Gene (Rat) : 316213SwissProt (Human): O43559SwissProt (Mouse) : Q91WJ0SwissProt (Rat) : Q52RG8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FRS3 expression in MCF7 (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of FRS3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.