Description: Rabbit polyclonal antibody to CUGBP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CUGBP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/50) Gene Symbol: CELF1 Alternative Names: BRUNOL2; CUGBP; CUGBP1; NAB50; CUGBP Elav-like family member 1; CELF-1; 50 kDa nuclear polyadenylated RNA-binding protein; Bruno-like protein 2; CUG triplet repeat RNA-binding protein 1; CUG-BP1; CUG-BP- and ETR-3-like factor 1; Deadenylation factor CUG-BP; Embryo deadenylation element-binding protein homolog; EDEN-BP homolog; RNA-binding protein BRUNOL-2Entrez Gene (Human): 10658Entrez Gene (Mouse) : 13046Entrez Gene (Rat) : 362160SwissProt (Human): Q92879SwissProt (Mouse) : P28659SwissProt (Rat) : Q4QQT3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CUGBP1 expression in HL60 (A), HeLa (B), NIH3T3 (C), Sol8 (D) whole cell lysates.
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Immunohistochemical analysis of CUGBP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.