Description: Rabbit polyclonal antibody to Dynactin p50 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Dynactin p50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DCTN2 Alternative Names: DCTN50; Dynactin subunit 2; 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitinEntrez Gene (Human): 10540Entrez Gene (Mouse) : 69654Entrez Gene (Rat) : 299850SwissProt (Human): Q13561SwissProt (Mouse) : Q99KJ8SwissProt (Rat) : Q6AYH5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Dynactin p50 expression in U87MG (A), EC9706 (B), mouse pancreas (C), rat heart (D) whole cell lysates.
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  Immunohistochemical analysis of Dynactin p50 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.