Description: Rabbit polyclonal antibody to PECI Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PECI. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ECI2 Alternative Names: DRS1; HCA88; PECI; Enoyl-CoA delta isomerase 2 mitochondrial; DRS-1; Delta(3),delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Diazepam-binding inhibitor-related protein 1; DBI-related protein 1; Dodecenoyl-CoA isomerase; Hepatocellular carcinoma-associated antigen 88; Peroxisomal 3,2-trans-enoyl-CoA isomerase; pECI; Renal carcinoma antigen NY-REN-1Entrez Gene (Human): 10455SwissProt (Human): O75521Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PECI expression in HEK293T (A), Hela (B) whole cell lysates.
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  Immunohistochemical analysis of PECI staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.