Description: Rabbit polyclonal antibody to EPAC1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EPAC1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RAPGEF3 Alternative Names: CGEF1; EPAC; EPAC1; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFIEntrez Gene (Human): 10411Entrez Gene (Mouse) : 223864Entrez Gene (Rat) : 59326SwissProt (Human): O95398SwissProt (Mouse) : Q8VCC8SwissProt (Rat) : Q9Z1C8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EPAC1 expression in A549 (A), SP2/0 (B), H9C2 (C) whole cell lysates.
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Immunohistochemical analysis of EPAC1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.