Description: Rabbit polyclonal antibody to PIAS3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PIAS3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PIAS3 Alternative Names: E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3Entrez Gene (Human): 10401Entrez Gene (Mouse) : 229615Entrez Gene (Rat) : 83614SwissProt (Human): Q9Y6X2SwissProt (Mouse) : O54714SwissProt (Rat) : O70260Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PIAS3 expression in rat lung (A), rat spleen (B) whole cell lysates.
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  Immunohistochemical analysis of PIAS3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.