Description: Rabbit polyclonal antibody to MPP9 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MPP9. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MPHOSPH9 Alternative Names: MPP9; M-phase phosphoprotein 9Entrez Gene (Human): 10198Entrez Gene (Mouse) : 269702SwissProt (Human): Q99550SwissProt (Mouse) : A6H5Y1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MPP9 expression in HeLa (A), NIH3T3 (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of MPP9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.