Description: Rabbit polyclonal antibody to CAPRI Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CAPRI. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RASA4 Alternative Names: CAPRI; GAPL; KIAA0538; Ras GTPase-activating protein 4; Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2Entrez Gene (Human): 10156Entrez Gene (Mouse) : 54153SwissProt (Human): O43374SwissProt (Mouse) : Q6PFQ7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CAPRI expression in NIH3T3 (A), PC12 (B), HEK293T (C), SGC7901 (D) whole cell lysates.
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Immunohistochemical analysis of CAPRI staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.