Description: Rabbit polyclonal antibody to GFR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GFR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RAPGEF5 Alternative Names: GFR; KIAA0277; MRGEF; Rap guanine nucleotide exchange factor 5; Guanine nucleotide exchange factor for Rap1; M-Ras-regulated Rap GEF; MR-GEF; Related to Epac; RepacEntrez Gene (Human): 9771Entrez Gene (Mouse) : 217944Entrez Gene (Rat) : 362799SwissProt (Human): Q92565SwissProt (Mouse) : Q8C0Q9SwissProt (Rat) : P83900Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GFR expression in HEK293T (A), A549 (B) whole cell lysates.
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Immunohistochemical analysis of GFR staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.