Anti-N4BP1 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of N4BP1 expression in AML12 (A), PC12 (B), HEK293T (C), A549 (D), A2780 (E) whole cell lysates.
Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Name	Anti-N4BP1 Antibody
Cat No:	CPA2415
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IH, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Rabbit polyclonal antibody to N4BP1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human N4BP1. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Gene Symbol: N4BP1

Alternative Names: KIAA0615; NEDD4-binding protein 1; N4BP1

Entrez Gene (Human): 9683

Entrez Gene (Mouse) : 80750

SwissProt (Human): O75113

SwissProt (Mouse) : Q6A037

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of N4BP1 expression in AML12 (A), PC12 (B), HEK293T (C), A549 (D), A2780 (E) whole cell lysates.
Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Lys-63-linked Ubiquitination by E3 Ubiquitin Ligase Nedd4-1 Facilitates Endosomal Sequestration of Internalized a-Synuclein

Journal J Biol Chem

IF 4.8

Application WB, ICC/IF

Reactivity

PMID 24831002

A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV

Journal eLife

IF 7.616

Application WB

Reactivity

PMID 30520725

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