Description: Rabbit polyclonal antibody to COX5A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: COX5A Alternative Names: Cytochrome c oxidase subunit 5A mitochondrial; Cytochrome c oxidase polypeptide VaEntrez Gene (Human): 9377Entrez Gene (Mouse) : 12858Entrez Gene (Rat) : 252934SwissProt (Human): P20674SwissProt (Mouse) : P12787SwissProt (Rat) : P11240Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of COX5A expression in MCF7 (A), SP2/0 (B), PC12 (C), Human liver (D) whole cell lysates.
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Immunohistochemical analysis of COX5A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.