Description: Rabbit polyclonal antibody to PAHT Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAHT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SLC22A6 Alternative Names: Solute carrier family 22 member 6; Organic anion transporter 1; Hoat1; Renal organic anion transporter 1; hROAT1; PAH transporter; hPAHT; SLC22A6; OAT1; PAHTEntrez Gene (Human): 9356SwissProt (Human): O95742Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PAHT expression in HeLa (A), mouse brain (B) whole cell lysates.
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Immunohistochemical analysis of PAHT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.