Description: Rabbit polyclonal antibody to TIF1 alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TIF1 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TRIM24 Alternative Names: RNF82; TIF1; TIF1A; Transcription intermediary factor 1-alpha; TIF1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24Entrez Gene (Human): 8805Entrez Gene (Mouse) : 21848SwissProt (Human): O15164SwissProt (Mouse) : Q64127Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TIF1 alpha expression in Jurkat (A), A549 (B), H446 (C), mouse liver (D) whole cell lysates.
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  Immunohistochemical analysis of TIF1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.