Description: Rabbit polyclonal antibody to B4GALT3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human B4GALT3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: B4GALT3 Alternative Names: Beta-1,4-galactosyltransferase 3; Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3Entrez Gene (Human): 8703Entrez Gene (Mouse) : 57370Entrez Gene (Rat) : 494342SwissProt (Human): O60512SwissProt (Mouse) : Q91YY2SwissProt (Rat) : Q6P768Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of B4GALT3 expression in Jurkat (A), HL60 (B), HeLa (C), COLO205 (D) whole cell lysates.
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  Immunohistochemical analysis of B4GALT3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.