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Anti-14-3-3 zeta Antibody CPA2245
  • Western blot analysis of 14-3-3 zeta expression in mouse liver (A), mouse spleen (B), rat liver (C), rat spleen (D) whole cell lysates.
  • Immunohistochemical analysis of 14-3-3 zeta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-14-3-3 zeta Antibody
Cat No: CPA2245
Source: Rabbit
Reactivity: H, M, R, B, C, P, S
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to 14-3-3 zeta
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol: YWHAZ
Alternative Names: 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1
Entrez Gene (Human): 7534
Entrez Gene (Mouse) : 22631
Entrez Gene (Rat) : 25578
SwissProt (Human): P63104
SwissProt (Mouse) : P63101
SwissProt (Rat) : P63102
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of 14-3-3 zeta expression in mouse liver (A), mouse spleen (B), rat liver (C), rat spleen (D) whole cell lysates.
  • Immunohistochemical analysis of 14-3-3 zeta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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