Anti-Vimentin (Phospho-S56) Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Vimentin (Phospho-S56) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S56 of human Vimentin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100) Gene Symbol: EZR Alternative Names: VIL2; Ezrin; Cytovillin; Villin-2; p81Entrez Gene (Human): 7430Entrez Gene (Mouse) : 22350Entrez Gene (Rat) : 54319SwissProt (Human): P15311SwissProt (Mouse) : P26040SwissProt (Rat) : P31977Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Vimentin (Phospho-S56) expression in H1688 (A) whole cell lysates. -
Immunohistochemical analysis of Ezrin (Phospho-T567) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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The calcineurin homologous protein-1 increases Na(+)/H(+) -exchanger 3 trafficking via ezrin phosphorylation