Description: Rabbit polyclonal antibody to USF2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USF2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: USF2 Alternative Names: BHLHB12; Upstream stimulatory factor 2; Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2Entrez Gene (Human): 7392Entrez Gene (Mouse) : 22282Entrez Gene (Rat) : 81817SwissProt (Human): Q15853SwissProt (Mouse) : Q64705SwissProt (Rat) : Q63665Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of USF2 expression in Hela (A), U2OS (B), mouse lung (C), mouse testis (D), rat lung (E) whole cell lysates.
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Immunohistochemical analysis of USF2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.