Description: Rabbit polyclonal antibody to UBE2A/B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human UBE2A/B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: UBE2A; UBE2B Alternative Names: UBE2A; RAD6A; Ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2B; RAD6B; Ubiquitin-conjugating enzyme E2 B; RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase BEntrez Gene (Human): 7320Entrez Gene (Mouse) : 22209; 22210Entrez Gene (Rat) : 81816SwissProt (Human): P49459; P63146SwissProt (Mouse) : Q9Z255; P63147SwissProt (Rat) : P63149Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBE2A/B expression in MCF7 (A), A549 (B), C6 (C), MEF (D) whole cell lysates.
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  Immunofluorescent analysis of UBE2A/B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).