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Anti-Tyrosinase Antibody CPA2200
  • Western blot analysis of Tyrosinase expression in A375 (A), Podocyte (B), Beas2B (C), PMVEC (D), BV2 (E) whole cell lysates.
  • Immunohistochemical analysis of Tyrosinase staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Tyrosinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product NameAnti-Tyrosinase Antibody
Cat No: CPA2200
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to Tyrosinase
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Tyrosinase. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene Symbol: TYR
Alternative Names: Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Entrez Gene (Human): 7299
SwissProt (Human): P14679
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Tyrosinase expression in A375 (A), Podocyte (B), Beas2B (C), PMVEC (D), BV2 (E) whole cell lysates.
  • Immunohistochemical analysis of Tyrosinase staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Tyrosinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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