Anti-TLE4 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of TLE4 expression in HeLa (A), Jurkat (B), HEK293T (C) whole cell lysates.
Immunohistochemical analysis of TLE4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Name	Anti-TLE4 Antibody
Cat No:	CPA2170
Source:	Rabbit
Reactivity:	H, M, R, C
Applications:	WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Rabbit polyclonal antibody to TLE4

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene Symbol: TLE4

Alternative Names: KIAA1261; Transducin-like enhancer protein 4

Entrez Gene (Human): 7091

Entrez Gene (Mouse) : 21888

Entrez Gene (Rat) : 25565

SwissProt (Human): Q04727

SwissProt (Mouse) : Q62441

SwissProt (Rat) : Q07141

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of TLE4 expression in HeLa (A), Jurkat (B), HEK293T (C) whole cell lysates.
Immunohistochemical analysis of TLE4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The corepressor tle4 is a novel regulator of murine hematopoiesis and bone development

Journal PLoS One

IF 3.7

Application WB

Reactivity Mouse

PMID 25153823

Tle4 regulates epigenetic silencing of gamma interferon expression during effector T helper cell tolerance

Journal Mol Cell Biol

IF 5.3

Application

Reactivity

PMID 24190972

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