Description: Rabbit polyclonal antibody to TGF alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TGF alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TGFA Alternative Names: Protransforming growth factor alphaEntrez Gene (Human): 7039Entrez Gene (Mouse) : 21802Entrez Gene (Rat) : 24827SwissProt (Human): P01135SwissProt (Mouse) : P48030SwissProt (Rat) : P01134Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of TGF alpha expression in U2OS (A), mouse heart (B), rat heart (C) whole cell lysates.
• 
  Immunohistochemical analysis of TGF alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.