Description: Rabbit polyclonal antibody to TEAD1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TEAD1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: TEAD1 Alternative Names: TCF13; TEF1; Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13Entrez Gene (Human): 7003Entrez Gene (Mouse) : 21676SwissProt (Human): P28347SwissProt (Mouse) : P30051Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TEAD1 expression in A431 (A), Raw264.7 (B), PC12 (C) whole cell lysates.
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Immunofluorescent analysis of TEAD1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development
The epigenetic control of E-box and Myc-dependent chromatin modifications regulate the licensing of lamin B2 origin during cell cycle