Description: Rabbit polyclonal antibody to TCF7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TCF7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TCF7 Alternative Names: TCF1; Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1Entrez Gene (Human): 6932Entrez Gene (Mouse) : 21414SwissProt (Human): P36402SwissProt (Mouse) : Q00417Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TCF7 expression in HEK293T (A), A549 (B), H1688 (C), mouse brain (D), mouse lung (E), rat brain (F), rat lung (G) whole cell lysates.
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Immunohistochemical analysis of TCF7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.