Description: Rabbit polyclonal antibody to TCF3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TCF3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TCF3 Alternative Names: BHLHB21; E2A; ITF1; Transcription factor E2-alpha; Class B basic helix-loop-helix protein 21; bHLHb21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3; TCF-3; Transcription factor ITF-1Entrez Gene (Human): 6929SwissProt (Human): P15923Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TCF3 expression in HeLa (A), BYDP (B) whole cell lysates.
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Immunohistochemical analysis of TCF3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis