Anti-STAT3 (Phospho-S727) Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to STAT3 (Phospho-S727) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/100) Gene Symbol: STAT3 Alternative Names: APRF; Signal transducer and activator of transcription 3; Acute-phase response factorEntrez Gene (Human): 6774Entrez Gene (Mouse) : 20848Entrez Gene (Rat) : 25125SwissProt (Human): P40763SwissProt (Mouse) : P42227SwissProt (Rat) : P52631Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAT3 (Phospho-S727) expression in HepG2 (A), A549 (B), LO2 (C), PC12 (D), 3T3L1 (E) whole cell lysates. -
Immunohistochemical analysis of STAT3 (Phospho-S727) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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