Description: Rabbit polyclonal antibody to STAT1 (Phospho-S727) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) Gene Symbol: STAT1 Alternative Names: Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84Entrez Gene (Human): 6772SwissProt (Human): P42224SwissProt (Mouse) : P42225Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAT1 (Phospho-S727) expression in A431 (A), NIH3T3 insulin-treated (B), Jurkat pervanadate-treated (C) whole cell lysates.
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Immunohistochemical analysis of STAT1 (Phospho-S727) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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