Description: Rabbit polyclonal antibody to SYT Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SYT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SS18 Alternative Names: SSXT; SYT; Protein SSXT; Protein SYT; Synovial sarcoma translocated to X chromosome proteinEntrez Gene (Human): 6760Entrez Gene (Mouse) : 268996SwissProt (Human): Q15532SwissProt (Mouse) : Q62280Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SYT expression in PC12 (A), NIH3T3 (B), Hela (C), MCF7 (D), HEK293T (E) whole cell lysates.
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Immunohistochemical analysis of SYT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.