Description: Rabbit polyclonal antibody to SOD1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: SOD1 Alternative Names: Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1Entrez Gene (Human): 6647Entrez Gene (Mouse) : 20655Entrez Gene (Rat) : 24786SwissProt (Human): P00441SwissProt (Mouse) : P08228SwissProt (Rat) : P07632Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SOD1 expression in mouse liver (A), mouse testis (B), rat liver (C), rat testis (D) whole cell lysates.
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Immunofluorescent analysis of SOD1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
PM2. 5 leads to adverse pregnancy outcomes by inducing trophoblast oxidative stress and mitochondrial apoptosis via KLF9/CYP1A1 transcriptional axis