Description: Rabbit polyclonal antibody to SNAP25 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SNAP25. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SNAP25 Alternative Names: SNAP; Synaptosomal-associated protein 25; SNAP-25; Super protein; SUP; Synaptosomal-associated 25 kDa proteinEntrez Gene (Human): 6616Entrez Gene (Mouse) : 20614Entrez Gene (Rat) : 25012SwissProt (Human): P60880SwissProt (Mouse) : P60879SwissProt (Rat) : P60881Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SNAP25 expression in HeLa (A), SKNSH (B), RAW264.7 (C), PC12 (D), rat brain (E) whole cell lysates.
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Immunohistochemical analysis of SNAP25 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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