Description: Rabbit polyclonal antibody to SGLT2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SGLT2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SLC5A2 Alternative Names: SGLT2; Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2Entrez Gene (Human): 6524Entrez Gene (Mouse) : 246787Entrez Gene (Rat) : 64522SwissProt (Human): P31639SwissProt (Mouse) : Q923I7SwissProt (Rat) : P53792Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SGLT2 expression in HEK293T (A), Hela (B), DLD (C), rat heart (D), rat liver (E), rat kidney (F) whole cell lysates.
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Immunohistochemical analysis of SGLT2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.