Description: Rabbit polyclonal antibody to GLUT3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GLUT3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SLC2A3 Alternative Names: GLUT3; Solute carrier family 2, facilitated glucose transporter member 3; Glucose transporter type 3, brain; GLUT-3Entrez Gene (Human): 6515SwissProt (Human): P11169Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GLUT3 expression in COLO205 (A), HepG2 (B) whole cell lysates.
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Immunohistochemical analysis of GLUT3 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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