Description: Rabbit polyclonal antibody to SRSF4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SRSF4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SRSF4 Alternative Names: SFRS4; SRP75; Serine/arginine-rich splicing factor 4; Pre-mRNA-splicing factor SRP75; SRP001LB; Splicing factor, arginine/serine-rich 4Entrez Gene (Human): 6429SwissProt (Human): Q08170SwissProt (Mouse) : Q8VE97Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SRSF4 expression in H1792 (A), Beas2B (B), SGC7901 (C), EC9706 (D), HEK293T (E), mouse kidney (F), rat heart (G) whole cell lysates.
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Immunohistochemical analysis of SRSF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Regulation of Mcl-1 by SRSF1 and SRSF5 in cancer cells