Description: Rabbit polyclonal antibody to S100-A10 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human S100-A10. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: S100A10 Alternative Names: ANX2LG; CAL1L; CLP11; Protein S100-A10; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; S100 calcium-binding protein A10; p10 protein; p11Entrez Gene (Human): 6281Entrez Gene (Mouse) : 20194Entrez Gene (Rat) : 81778SwissProt (Human): P60903SwissProt (Mouse) : P08207SwissProt (Rat) : P05943Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of S100-A10 expression in HT29 (A), HeLa (B), A431 (C) whole cell lysates.
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Immunohistochemical analysis of S100-A10 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.