Description: Rabbit polyclonal antibody to Radixin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Radixin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RDX Alternative Names: RadixinEntrez Gene (Human): 5962Entrez Gene (Mouse) : 19684SwissProt (Human): P35241SwissProt (Mouse) : P26043Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Radixin expression in HEK239T (A), Hela (B), HGC27 (C), mouse kidney (D), mouse testis (E), rat kidney (F), rat testis (G) whole cell lysates.
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Immunohistochemical analysis of Radixin staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.