Description: Rabbit polyclonal antibody to RAP1GAP Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RAP1GAP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RAP1GAP Alternative Names: KIAA0474; RAP1GA1; Rap1 GTPase-activating protein 1; Rap1GAP; Rap1GAP1Entrez Gene (Human): 5909Entrez Gene (Mouse) : 110351SwissProt (Human): P47736SwissProt (Mouse) : A2ALS5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RAP1GAP expression in mouse kidney (A) whole cell lysates.
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Immunohistochemical analysis of RAP1GAP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.