Anti-Patched Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Patched Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Patched. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTCH1 Alternative Names: PTCH; Protein patched homolog 1; PTC; PTC1Entrez Gene (Human): 5727Entrez Gene (Mouse) : 19206SwissProt (Human): Q13635SwissProt (Mouse) : Q61115Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Patched expression in HeLa (A), Ramos (B), MCF7 (C), NIH3T3 (D), PC12 (E) whole cell lysates. -
Immunohistochemical analysis of Patched staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival
Fuz Regulates Craniofacial Development through Tissue Specific Responses to Signaling Factors
Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron
AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5
Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A