Description: Rabbit polyclonal antibody to PKC zeta (Phospho-T560) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T560 of human PKC zeta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PRKCZ Alternative Names: PKC2; Protein kinase C zeta type; nPKC-zetaEntrez Gene (Human): 5590Entrez Gene (Mouse) : 18762Entrez Gene (Rat) : 25522SwissProt (Human): Q05513SwissProt (Mouse) : Q02956SwissProt (Rat) : P09217Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PKC zeta (Phospho-T560) expression in HEK293T (A), Hela (B), H446 (C), mouse kidney (D), mouse testis (E), rat kidney (F), rat testis (G) whole cell lysates.
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Immunohistochemical analysis of PKC zeta (Phospho-T560) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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